To study proteins from sample and detect their presence in food sample

Aim: To study proteins from sample and detect their presence in food sample. 

Material required: Test-tube, test-tube stand, test tube holder, glass rod, burner etc.

Theory: Proteins are high molecular mass, long chain polymers composed of a amino acid. Amino acids are molecule that have both -NH2 and –COOH group.

 

S.N.	EXPERIMENT	OBSERVATION	INFERENCE
1.	Biuret test: Sample + NaOH + Dil. CuSO4 solution	Bluish violet colour appears	Protein is present
2.	Xanthoprotein test: Sample + few drops of conc. HNO3 + D	Yellow ppt.	Protein is present
3.	Million’s test: Sample + 2 drops of millions reagent + D	White ppt which changes to brick red on boiling	Protein is present
4.	Ninhydrin test: Protein sample + Few drops of ninhydrin solution + Boil the contents for 1 minute	Blue colour appear	Protein is present

 

Result: Protein is present in given sample of food.