To study fat in pure form and to detect into presence in given food sample

Aim: To study fat in pure form and to detect into presence in given food sample 

Material required: Test-tube, test-tube stand, test tube holder, burner etc.

Theory: Fats and oil are esters of long chain fatty acids and glycerol and thus also called glycerides. Fats contains saturated fatty acids while oil contain unsaturated fatty acids.

 Observation Table-

S.N.	EXPERIMENT	OBSERVATION	INFERENCE
1.	Spot test: put a small amount of sample on a filter paper and press with another filter paper	Translucent spot appears on filter paper	Fat is present
2.	Acrolein test: Take a few drops of sample in a test tube. Add few drops of potassium bisulphite to it and Heat.	Irritating smell appears due to the form of acrolein vapours.	Fat is present
3.	Solubility test: Take a small amount of sample in 3 test tubes. Add water, alcohol, and chloroform in 1, 2, 3 test tube respectively.	Sample does not dissolve in H2O but is soluble in alcohol on heating and soluble in chloroform.	Fat is present

 

Result: Fat is present in given sample of food.